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A Look at Pregnancy Rates when Dosing By Progressive Motility

Wednesday, May 12, 2010

At 11:45 am on July 11th, 2010 Advanced Agricultural Technologies will present the results of our 2 year Progressive Motility fertility study for the first time.  It will happen at the American Dairy Science Association annual meeting in Denver, Colorado (taking place the 11th through the 15th).  Please join us there and see how our technology will change the industry.  Here is an abstract of the presentation:

The Impact on Pregnancy Rates in Dairy Cattle Artificially Inseminated with Semen Prepared by Number of Progressively Motile Sperm.

Rabinovitch^{1}^; U. Shalit^{1}^; M. Deutsch^{1}^;Y. Zeron^{2}^;P.Chenoweth^{3}^Medical Electronic Systems^{1}^; Sion A.I. Company, Israel^{2}^; CharlesSturt University, Australia^{3}^

The trial objective was to evaluate the pregnancy rates of dairy cattle inseminated with varying amounts of progressively motile sperm, post thaw (PMPT).  In phase 1, straws were prepared to contain specific numbers of progressively motile sperm, post thaw.  In phase 2, cows were inseminated and pregnancy rates were analyzed.  All semen testing and dosing was performed at an operating AI stud facility (SION A.I. Company) using an SQA-Vb, automated sperm quality analyzer for Bulls (Medical Electronic Systems).  Forty ejaculates collected from four bulls were split into four groups and 2000 straws per group were targeted to contain: Group A, B and C; 1.5, 3.5 and 7.0 million PMPT sperm/straw respectively. The target for group D (Control) was 15.0 million total sperm per straw per the AI stud’s routine.  228 straws from groups A, B and C were quality control tested based on mean (+/- SE) values for PMPT sperm and demonstrated:  (A) 1.5 (+/- 0.05); (B) 3.0 (+/- 0.09) and (C) 6.2 (+/- 0.18) million. 76 straws from Group D (Control) established a mean (+/- SE) value of 13.6 (+/- 0.15) million. 6,494 cows in over 500 farms were blindly inseminated between January and April 2009.  At approximately 42 days post-insemination, pregnancy rates (per cow per cycle) were determined per rectal palpation by group as follows: (A) 34.2%; (B) 39.3%; (C) 43.2%; (D) 38.6%. The relative difference in pregnancy rates for groups A, B and C versus the control (D) were: -11.4%, 1.8% and 11.9%, respectively.  Pregnancy rates in groups A, B and C correlated to the number of progressively motile sperm (r=0.96).  It is concluded that the number of progressively motile sperm per AI dose significantly impacts subsequent bovine pregnancy rates. Further, it is possible to accurately produce AI straws based on the number of PMPT sperm. Use of these findings could help improve bovine AI reproductive performance while allowing more effective utilization of superior bulls.

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